By applying BRAF kinase inhibitors to EC cell lines with BRAF gene mutation, we can detect whether the expression of differentiation markers is decreased, whether the tumor cells have proliferation arrest, whether the activity of BRAF protein kinase is down regulated, and whether the relative expression of nuclear related genes is decreased, To determine whether the invasion and metastasis of the cells are reduced, so as to obtain a more accurate relationship between BRAF mutation and the occurrence and development of EC. The gene discussed is WEE1; the disease is neoplasm.