Differential analysis of the osteosarcoma-related microarray data GSE12805 containing tumor tissues (n = 40) and normal tissues (n = 13) was performed using R language with |log2FC|> 1 and p-value < 0.05 as threshold, with results indicating that PTBP1 was upregulated in osteosarcoma (Figure 1A). This evidence concerns the gene PTBP1 and osteosarcoma.