Western blot analysis revealed an increased expression of PTBP1 in parental U2OS, MG63, and HOS cells in the presence of PTBP1, and decreased PTBP1 expression in DXR-resistant osteosarcoma cells in the absence of PTBP1 (p < 0.05) (Figure 3B). Here, PTBP1 is linked to osteosarcoma.