The results revealed enhanced cell viability and increased IC50 in parental U2OS, MG63, and HOS cells in the presence of PTBP1, while a contrasting trend was identified in the DXR-resistant osteosarcoma cells in the absence of PTBP1 (p < 0.05) (Figure 3C, 3D). The gene discussed is PTBP1; the disease is osteosarcoma.