PTBP1 and osteosarcoma: In addition, we identified that siRNA-mediated knockdown of GLUT3 along with overexpressed PTBP1 reversed the effects of overexpressed PTBP1 in U2OS/DXR cells, suggesting that PTBP1 or GLUT3 facilitated chemoresistance of osteosarcoma cells to DXR by enhancing aerobic glycolysis (Figure 4D).