YTHDF1 and hepatocellular carcinoma: However, similar YTHDF1 levels were obtained with and without LW6 treatment in CMV-driven FLAG-YTHDF1-overexpressing HCC cells under hypoxia, indicating that HIF-1α-mediated YTHDF1 induction had no effect on the CMV promoter, but did affect expression driven by the endogenous promoter (Fig. 2i, j).