TICRR and nevoid basal cell carcinoma syndrome: Using 2D analysis, previous studies have analyzed replication intermediates in cells with reduced origin firing efficiency, including the rARSΔ-3 strain, the orc4Y232C mutant that mimics the mutation carried by patients with Meier–Gorlin syndrome, and cells depleted of the replication initiation factors Sld2 and Sld3 (28, 32, 33).