Nlrp6-/- mice showed no alteration in the intestinal permeability after B. abortus infection, but surprisingly, when the microbiota was depleted in these mice, there was a significant increase in the B. abortus CFU load in the liver after oral infection with B. abortus (Fig 6B and 6C), indicating that the resistant phenotype observed in Nlrp6-/- infected mice was dependent on the gut microbiota composition. The gene discussed is NLRP6; the disease is infection.