PKD2 and polycystic kidney disease: Embryos with mutations in pkd2 (polycystic kidney disease 2), which is required for Kupffer’s Vesicle function,42 exhibit defects in left–right asymmetry including a disruption to normal leftward displacement of the heart tube,42 while spaw mutants lack asymmetric Nodal expression prior to asymmetric organ morphogenesis resulting in midline positioning of the heart tube.7