Some findings imply that high-fructose diets can enhance the prevalence of NAFLD, lipid accumulation, expression of fatty acid synthase (FASN), hepatic weight, but inhibit the expression and phosphorylation of IRS-1/Akt/endothelial nitric oxide synthase (eNOS) signaling intermediates in the liver. Here, NOS3 is linked to metabolic dysfunction-associated steatotic liver disease.