Rho/ROCK/LIMK/cofilin is one of the Rho GTPase pathways, the inhibition of Rho/ROCK/LIMK/cofilin pathway resulted in the destroy of F-actin stabilization and redistribution of cytoplasmic actin via inhibition of cofilin phosphorylation, which promoted EMT process as well as gastric cancer metastasis. This evidence concerns the gene LIMK1 and gastric cancer.