CHMP2B and myocardial infarction: Therefore, this study aimed at: (i) determining the association between myocardial CHMP2B levels, myocardial autophagy function, and myocardial injury during MI/R; (ii) establishing whether the accumulation of CHMP2B impairs myocardial autophagy and aggravates MI/R injury; and (iii) evaluating whether metformin protects against MI/R injury by regulating CHMP2B and autophagic flux.