Other studies showed that lncRNA DICER1-AS1 expression was up-regulated in osteosarcoma cells, while knockdown of DICER1-AS1 could inhibit the proliferation, migration, and invasion of osteosarcoma cells, and reduce the protein expression levels of ATG5, LC3, and Beclin1, indicating that knocking down DICER1-AS1 can inhibit autophagy of osteosarcoma cells (27). This evidence concerns the gene ATG5 and osteosarcoma.