In order to reveal the mechanism of HOXD11 leading to poor prognosis in glioma patients, GSEA was used to indirectly reveal the signal pathway activated by HOXD11, which indicated that HOXD11 is significantly enriched in cancer-related cell signaling pathways, such as cell cycle, DNA replication, ECM receptor interaction, and focal adhesion. This evidence concerns the gene HOXD11 and central nervous system cancer.