The anti-tumor activity of GUCY2C-specific CAR-T cells has been confirmed in both human and syngeneic CRC xenograft models which used murine T cells, but CARs produced from 5F9 scFv (GUCY2C-scFv) didn't show cross-reactivity with murine GUCY2C, limiting the quantification of intestinal toxicity in mice models 25. Here, GUCY2C is linked to colorectal carcinoma.