For example, 1) intestine Treg can be classified into three subsets including GATA3+Helios+ (Nrp1+, thymic origin) (107); RORgt+Helios− (microbial immunity), and RORgt−Helios− (against dietary antigens); 2) contrary to obese animals, depletion of VAT-Treg in aged animals improves the metabolic parameters and rescues aging-induced insulin resistance (107); and 3) brown adipose tissue Treg help in thermogenesis (107). The gene discussed is GATA3; the disease is Insulin resistance.