A preliminary RNAseq analysis of BCF000174 compared to two non-CF donors (WP11, WP21) cultured and analyzed in parallel confirms the expression of all enzymes known to be involved in ceramide synthesis, including ceramide synthase isozymes, PUFA elongases (ELOVL), and fatty acid desaturases (FADS), but the data do not readily explain the observed lipid imbalance in CF HBEC-ALI (Scholte et al., 2021). Here, TLCD3B is linked to acute respiratory distress syndrome.