The BHK21-hACE2 cells with high expression level of human angiotensin-converting enzyme 2 (hACE2) need only 6 h to proliferate one generation, which support efficiently infection of pseudovirus and infection of pseudovirus can be detected by fluorescence 12 h after infection, enabling the assay time-saving for high-throughput screening (Xiong et al., 2020). This evidence concerns the gene ACE2 and infection.