Since PSD-95 is the major scaffolding protein at the excitatory postsynaptic density, a potent regulator of synaptic strength, and is known to be required for retrieval and stability of fear memory (Fitzgerald et al., 2015), we detected PSD-95 as an indirect synaptic marker in total protein samples extracted from the hippocampi of 3xTg-AD mice after TFC. This evidence concerns the gene DLG4 and Alzheimer disease.