As shown in Figure 3, the percentage of entries in the open arm [t(18) = 3.908, p = 0.0010, Figure 3E], the percentage of time spent in the open arms [t(18) = 2.547, p = 0.0202, Figure 3F], and the percentage of distance traveled in the open arm of the maze [t(18) = 3.474, p = 0.0027, Figure 3D] were significantly lower in 21-week-old Slit2-Tg mice than in 21-week-old WT mice, indicating increased anxiety-like behavior in 21-week-old Slit2-Tg mice. This evidence concerns the gene SLIT2 and Anxiety.