Compared with 21-week-old WT mice, the percentage of time in the open arms, distance on open arms and the percentage of entries into open arms of the maze, and the percentage of time spent at the center and the percentage of distance traveled at the center of the open field were significantly decreased in the Slit2-Tg mice of the same age, illustrating that the adult Slit2-Tg mice exhibited increased anxiety-like behavior. Here, SLIT2 is linked to Anxiety.