Compared to the pre-infection (QFT−) timepoint, recent QFT+ conversion was associated with a decrease in M.tb lysate-specific, early-differentiated IL-2-only TSCM/TCM (cluster 2565) and TNF-only TSCM/TCM (cluster 2570) cell subsets, and an increase in proportions of IFN-γ+TNF+IL-2+ (cluster 2559/66) and IFN-γ+TNF+ TE (cluster 2567) cells (Fig. 4a). The gene discussed is TNF; the disease is infection.