Both CSF p‐NfH and NfL displayed a high accuracy for ALS diagnosis when compared to HC (AUC 0.939, P < .0001, and AUC 0.995, P < .0001, respectively) (Figure 3A, B), and the best cut‐off values were 0.652 ng/mL for CSF p‐NfH (sensitivity 87.5%, specificity 100%) and 1261 pg/mL for CSF NfL (sensitivity 93.75%, specificity 100%). This evidence concerns the gene NEFH and amyotrophic lateral sclerosis.