VANGL2 and spina bifida: To corroborate this finding in an independent model, we used Ghrl3Cre to mosaically delete neuroepithelial Vangl2. Ghrl3Cre deletion of Vangl2Fl/Fl produces late depletion of neuroepithelial VANGL2 and distal spina bifida, without requiring pre-deletion of a Vangl2 allele14.