Although the latest study23 showed that CDKN2B-AS1 can function as a ceRNA to regulate cyclin-D1/D2 expression, resulting in RCC aggressiveness, by using cell cytoplasm/nuclear fractionation assays, we found that CDKN2B-AS1 is mainly localized in the nucleus, as in previous reports on other tumors30,37,38, and indicating that CDKN2B-AS1 might regulate NUF2 expression via a transcriptional mechanism. This evidence concerns the gene CDKN2B and renal cell carcinoma.