In 11 cohorts, only pregnant women with positive RT-PCR for ZIKV in serum or urine were included [23,25–30,33,34,36,39] and ten cohorts included pregnant women with RT-PCR confirmed infection for ZIKV in serum or urine and those with probable infection with IgM measurement with PRNT for ZIKV [18–22,24,35,37,38,40]. This evidence concerns the gene CD40LG and infection.