To further validate the results of bioinformatics analysis, the gene expression levels of the 12 hub genes from PPI network (ALOX5AP, BCL2A1, BTK, CYBB, NCF2, HCK, HK3, CSF1R, PLEK, CD300A, ITGB2, and FPR1) in nasal polyps from CRSwNPs and nasal mucosa from healthy controls were determined by RT-qPCR. Here, HCK is linked to Nasal polyposis.