In the present study, we dissected the impact of low glutamine on regulating the expression of G-CSF and GM-CSF in mouse mammary tumor cells, we elucidated the role of the bone marrow niche in the mobilization of myeloid precursors in breast cancer, and we provide evidence to mechanistically link microenvironmental glutamine depletion in cancer to the expansion and mobilization of MDSCs. This evidence concerns the gene CSF3 and breast cancer.