We also found that BMP9 could: 1) alleviate obesity, liver steatosis and macrophage infiltration; 2) improve glucose tolerance and insulin sensitivity; 3) downregulate genes related to lipid and glucose metabolism, as well as inflammatory responses; 4) and reshape chromatin accessibility in HFD-induced NAFLD mice. The gene discussed is INS; the disease is obesity due to melanocortin 4 receptor deficiency.