Time-dependent chemosensitizing activity was evidenced by the augmentation of cisplatin-induced apoptosis detected with Hoechst33342/PI costaining (Fig. 3b,c) and annexin V-FITC/PI assay through flow cytometry (Fig. 3d,e) of human lung cancer cells pretreated with 5 μg/ml of purified 0.4 M NaCl peptide from L. squarrosulus extracts for 6–24 h. This evidence concerns the gene ANXA5 and lung carcinoma.