Since the post-mortem and neuroimaging studies reveal that cholinergic dysfunction is observed in individuals with schizophrenia17,18, we investigated the level of Hrh1 mRNA in cholinergic neurons by RNAscope in situ hybridization (ISH) in brain samples from adult patients with schizophrenia (Supplementary Data 1). This evidence concerns the gene HRH1 and schizophrenia.