When clozapine N-oxide (CNO, 1 mg/kg) was intraperitoneally administered to activate BF cholinergic neurons, behavioral abnormalities were completely ameliorated in the ChAT-Cre;Hrh1fl/fl mice, including disrupted PPI, social impairments, anhedonia-like behavior, and cognitive impairments (Fig. 5b–g). This evidence concerns the gene CHAT and Cognitive impairment.