When CNO (i.p. 1 mg/kg) was administrated for 1 week to reduce BF cholinergic activity, ChAT-Cre mice expressing hM4Di showed disrupted PPI, social impairments, anhedonia-like behavior, and cognitive impairments, but no change in locomotion, which is similar to behavioral symptoms manifested in ChAT-Cre;Hrh1fl/fl mice (Fig. 6b, d–i). Here, CHAT is linked to Cognitive impairment.