HRH1 and schizophrenia: To further demonstrate whether H1R in cholinergic neurons is associated with the behavioral deficits in an experimental schizophrenia model, and whether H1R is an appropriate therapeutic target, ChAT-Cre mice were injected with AAV-CAG-FLEX-Hrh1-GFP into the BF to overexpress H1R in BF cholinergic neurons, followed by treatment with MK-801 for 2 weeks (0.2 mg/kg/d) (Fig. 7b).