IFNG and neoplasm: To investigate whether iTreg are tumor-reactive, we also performed single-cell sequencing of sorted MAMI-specific Treg and Tconv from autologous blood using either mam34–48-labeled HLA Class II tetramers or the IFNγ-secretion assay targeting MAMI-reactive IFNγ+Teff (Supplementary Fig. 1e, f and 5c, d).