Importantly, cluster 1 was also marked by RTKN2, which was recently identified in healthy donor blood-derived Treg82, and the tumor-infiltrating Treg markers LAYN, MAGEH1, and CCR8, which associates with increased proliferative potential and suppressive capacity but most importantly poor prognosis in breast cancer, NSCLC, and CRC patients30,83. This evidence concerns the gene LAYN and breast cancer.