Among tumor-infiltrating CD4+ cells, we identified CD25+CD127- Treg, CD25−CD127-/+ Tconv but also a third CD25+CD127+ subset designated ActTconv (Fig. 3a, Supplementary Figs. 1d and 5a, b), as CD25 is upregulated on TC upon TCR stimulation52, and the vast majority lacked CD45RA expression, suggesting previous antigen experience. The gene discussed is CD4; the disease is neoplasm.