Tissue morphology (Fig. 1b, Supplementary Fig. 1a), presence of luminal markers PSA, NKX3.1, AR, CK8 expression, and absence of CK5+ basal cells (Fig. 1b, Supplementary Fig. 1b–d) indicated stable luminal epithelial morphology among the primary TUR-P tumor (T1), the PNmet, and PDX1–6 passages. Here, NKX3-1 is linked to neoplasm.