Interestingly, while Tregs and neutrophils correlated to TN subtype, and T lymphocytes, Tregs, macrophages, and neutrophils in primary tumors correlated to ER-negative breast cancer, no correlations to molecular subtype or other clinicopathological factors could be seen for either FOXP3 or CD3 in distant metastases, suggesting that Tregs in distant metastases could be a prognostic factor for patients with metastatic breast cancer independent of breast cancer subtype. This evidence concerns the gene FOXP3 and breast carcinoma.