Having understood the clinicopathological significance of the CALM1 and EGFR in vivo in ESCC, therefore we hypothesized that knockout of CALM1 and treatment with EGFR inhibitor (Afatinib) could markedly impair the proliferation and apoptosis in vitro in ESCC cell lines. The gene discussed is CALM1; the disease is esophageal squamous cell carcinoma.