Next, to investigate the combined effect of knock-out of CALM1 and EGFR inhibitor that exerted over proliferation of ESCC cells, we carried out Transwell assay and Wound-healing assays after KYSE150 and Eca109 were transfected with lentiviral-based knockout of CALM1. Here, EGFR is linked to esophageal squamous cell carcinoma.