To elucidate the possible role of Tregs in AIH pathogenesis, we first analyzed thymic and peripheral Treg generation in Alb-iGP_Smarta mice (Figure 6A) and found that thymic Treg induction was negligible, whereas peripheral Treg induction was significantly increased in Alb-iGP_Smarta mice (6.1% splenic Tregs), as compared with Smarta mice that do not feature the GP61–80 autoepitope (1.8% splenic Tregs; Figure 6B; P = 0.068). This evidence concerns the gene ALB and autoimmune hepatitis.