To do this, the RNAscope® Duplex Assay [56] was used to analyze the expression of ASPN in the stroma adjacent to benign glands, Gleason grade 3, Gleason grade 4 cribriform, and Gleason grade 4 noncribriform prostate cancer in radical prostatectomy specimens in dual combination with other markers expressed in fibroblast lineage cells: FAP, THY1, ENG, NT5E, TNC, and PDGFRβ. This evidence concerns the gene ASPN and prostate cancer.