Samples were additionally quantified for dual expression of ASPN and ENG. The percentages of ASPN+ENG+ cells and ASPN+ENG− cells were significantly lower than ASPN−ENG+ cells in the stroma adjacent to benign prostate (p < 0.0001); however, it was only the ASPN+ENG+ cells that were enriched in the tumor microenvironment with the most pronounced increase in Gleason grade 4 prostate cancers containing either cribriform and noncribriform morphologies (Figure 3E and supplementary material, Table S5). This evidence concerns the gene ASPN and Familial prostate cancer.