While the overall percentage of NT5E+, TNC+, and PDGFRβ+ cells did not vary in the prostate tumor microenvironment compared to benign prostate, subsets of NT5E+, TNC+, and PDGFRβ+ cells detected in benign prostate stroma were differentially altered in the stroma adjacent to Gleason grade 4 cribriform prostate cancer. Here, TNC is linked to male reproductive organ cancer.