HPSE expression was closely correlated with Edmondson’s classification (P = 0.0318), AJCC staging of HCC (8th edition) (P = 0.0375), multiple tumor foci (MTF) (P = 0.0093), and microvascular invasion (MVI) (P = 0.0043). This evidence concerns the gene HPSE and hepatocellular carcinoma.