These protective effects of NLRP3 deficiency on memory after zinc deficiency were independent of plaque burden or microglia activation as there was no significant difference in these parameters in APP/PS1 mice with or without nlrp3 (three-way interaction term: plaque F(1,46) = 1.15, p = 0.290; microglia F(1,46) = 0.49, p = 0.488) (Fig. 5B–D). This evidence concerns the gene NLRP3 and Zinc deficiency.