As our bioinformatics analysis in TCGA tumors showed negative correlations of miR-34a-5p with the expression of epithelial-to-mesenchymal transcription factors such as SNAI2, ZEB1, ZEB2, and TWIST2 and presented in Fig. 1g, we assessed expression changes of epithelial and mesenchymal markers upon miR-34a-5p overexpression in HNSCC cell lines. This evidence concerns the gene TWIST2 and head and neck squamous cell carcinoma.