EEA1 and viral infectious disease: While the number and distribution of late endosomes (LAMP1 punctate staining) was not affected, EEA1 labeling revealed significantly less early endosomes following viral infection (S2C and S2D Fig) and GFP-tagged Sec61β, used as a marker of ER morphology, remained similarly distributed in infected and uninfected cells (S2E Fig) suggesting that no major ER morphology changes occur during H1N1 infection.