PAICS and colon adenoma: In the MSK cohort analyzed on Affymetrix microarray, as shown in Fig 1B, we confirmed that PAICS was significantly overexpressed in colon adenomas and CRCs compared to those of normal mucosa (P<0.0001 and P<0.0001, respectively), and again we observed a significant decreasing trend of PAICS expression with progressive disease stages in primary tumors (r = -0.19, P<0.01).