To test this, we started by determining the effect of small interfering RNA (siRNA)-mediated depletion of the critical NMD component UPF1 on spontaneous EBV reactivation in the human gastric carcinoma cell line AGS-EBV, which harbors recombinant EBV Akata-BX1 that encodes green fluorescent protein (GFP) under control of the lytic BXLF1 promoter [32]. The gene discussed is UPF1; the disease is gastric carcinoma.