RNF167 and neoplasm: These differences persisted even with arginine concentration reaching 50 μM indicating that the combined effects of AKT1 phosphorylation and RNF167-targeting degradation had a stronger role than arginine inhibition of CASTOR1 in regulating mTORC1 activation, particularly at a condition with a low concentration of arginine, which is common in tumor microenvironment (Fig. 4h).