ACE2 and infection: The ultrapermissive 293T-ACE2+TMPRSS2 F8-2 clone in particular can support the use of a standardized VNA at the scale needed for screening entry inhibitors, vaccine samples, donor plasma, etc. Our standardized CoV2pp production lot from a single lab, 30 10-cm dishes, was sufficient for ∼12,000 infections/week when the assay was performed in a 96-well format.