Meanwhile, gedatolisib resistance were also observed when administrated in ABCB1- and ABCG2-gene-transfected HEK293 cells, while not in parental HEK293/pcDNA3.1 cell line, further verifying that overexpression of ABCB1 and ABCG2 confer resistance to gedatolisib in CRC cells. Here, ABCG2 is linked to colorectal carcinoma.