PIVOT analysis revealed eight transcription factors (CHD8, CTBP1, E2F1, E2F4, E4F1, TP53, TP53BP1, and ZNF143) in the turquoise module (P < 0.01) and four (CEBPA, DEDD, IRF8, and SPI1) in the blue module as significantly correlated with AML (Figure 5A, 5B). Here, E2F1 is linked to acute myeloid leukemia.