To identify which out of the 95 genes we identified in the Drosophila eye screen represent true dependencies for RB1-deficient cells, we interrogated the effects of the depletion of their human orthologs on the proliferation of human cancer cell lines from the COLT, DRIVE, AVANA, and DEPMAP projects based on their RB1 status (Fig 2A). Here, RB1 is linked to cancer.