In this study, we evaluated the expression of target genes of NFkB pathway (MYC, BCL2), the essential transcriptional regulator in hematopoiesis (LMO2), the checkpoint regulation pathway MGMT, the transcription factor POU2F1, and the immune checkpoint gene PDCD1 in patients with PCNSL. This evidence concerns the gene NFKB1 and primary central nervous system lymphoma.