PALD1 and neoplasm: As an initial approach to establish a link between PDAC-associated ECM alterations and expression of palladin isoforms 3 and 4 (iso3 and iso4), we used multi-photon microscopy to acquire second harmonic generation (SHG) of polarized light, and evaluated the topographic characteristics of bundled collagen in three matched PDAC and tumor adjacent human surgical specimens.