To further corroborate whether ASTILCS expression is critical for HCC cell survival, we measured its expression in HUH7 HCC cells transfected with the three most efficient shRNAs from the library (Supplemental Fig. 5) and observed dosage-dependent decrease in HCC cell survival (Figs. 3C and 2A). The gene discussed is ASTILCS; the disease is hepatocellular carcinoma.