To investigate the effector functions of memory Nr2f6-deficient CD8+ T cells, we stimulated splenocytes ex vivo on d70 post infection either in an antigen-independent manner using phorbol 12,13-dibutyrate (PDBu) and ionomycin (P/I) or in an antigen-specific manner with the SIINFEKL (N4) peptide. Here, CD8A is linked to infection.