Together, these above results indicated that SChLAP2 promoted tumorigenesis of prostate cancer in vitro and in vivo through interacting with EZH2 to mediate a methylation-modification regulation in miR-340-5p/miR-143-3p/miR-145-5p and DNMT3a signaling pathways. This evidence concerns the gene EZH2 and prostate carcinoma.