UBE2S and cancer: The pipeline for variant analysis of cancer WES data was previously described.32 Single nucleotide variations, insertions, and deletions (indels) were analyzed using the three-caller pipeline to identify somatic mutations through the comparison of variants identified in tumor exome data sets against germline variants and dbSNP in paired adjacent samples.32 A somatic mutant UBE2S with two different mutation sites (p.Gly57Ala and p.Lys63Asn) were identified in a single patient.